Journal: Microbiome

Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

doi: 10.1186/s40168-025-02099-6

Figure Lengend Snippet: Taxonomic identification of yeasts isolated from larvae and water samples collected from Ae. albopictus breeding sites. Field sampling was performed in September–October 2019 within a community garden localized in Saint Priest (Metropolis of Lyon, France). The 10 waterborne yeasts colonizing the gut of Ae. albopictus larvae were selected to evaluate their capacity to secrete uricase and impact mosquito development

Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

Techniques: Isolation, Sampling

Journal: Microbiome

Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

doi: 10.1186/s40168-025-02099-6

Figure Lengend Snippet: Uric acid recycling pathway present in R. mucilaginosa strains, including the one isolated from both Ae. albopictus larvae and water-breeding sites. An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling. Some of these genes (blue arrows) were identified as expressed in an available transcriptome , and their presence in the genome of the strain used in this study was confirmed by PCR (red stars)

Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

Techniques: Isolation

Journal: Microbiome

Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

doi: 10.1186/s40168-025-02099-6

Figure Lengend Snippet: Impact of acetohydroxamic acid-mediated urease inhibition on the development time of gnotobiotic Ae. albopictus larvae associated with uricolytic/ureolytic ( R. mucilaginosa ) and non-uricolytic/ureolytic ( M. asiatica ) yeast species. To confirm the involvement of the yeast uric acid recycling pathway in larval development, we inhibited it by targeting urease, the only enzyme in the yeast recycling pathway absent from the Asian tiger mosquito genome. The impact of this inhibition on the development time of gnotobiotic mosquitoes ( n = 60 per infection status) was assessed using an inhibitor concentration of 2.5 mM. Prior to this experiment, we confirmed that this inhibitor concentration effectively inhibited R. mucilaginosa urease activity without affecting yeast growth or axenic larval development. Statistically significant differences between groups were identified with the Tukey post hoc tests. Columns labelled with an asterisk (*) are significantly different with a p -value < 0.05. ns, not significant

Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

Techniques: Inhibition, Infection, Concentration Assay, Activity Assay

Journal: Microbiome

Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

doi: 10.1186/s40168-025-02099-6

Figure Lengend Snippet: Impact of yeast species and their cell concentrations on oviposition behavior of Ae. albopictus gravid females. The impact of six yeast species ( R. mucilaginosa , A. pullulans , H. uvarum , M. pulcherrima , T. delbrueckii , and M. asiatica ) at two cell concentrations (3.10 2 and 3.10 5 cells.mL −1 ) on the oviposition behavior of gravid females was evaluated using two distinct and complementary laboratory bioassays. Oviposition preference bioassays (corresponding to A & B ) consisted of small-cage three-choice oviposition tests, in which each single gravid female ( n = 24) was allowed to choose between three oviposition sites (double-beaker systems) providing oviposition substrates containing waterborne substances (sterile water or sterile water inoculated with yeasts) and olfactory stimuli (volatile organic compounds emitted by sterile CYM medium or yeast cultures). A Total number of eggs laid by each female per cage, regardless of the selected oviposition site. The pie charts represent the percentages of females that have laid eggs (shown in gray) or have not laid eggs (shown in black). B Number of eggs laid by each female in each oviposition site during three-choice oviposition tests. These oviposition preference bioassays were used to assess short-range attractiveness or repellency of volatile compounds emitted by yeasts but only because they were systematically paired with oviposition stimulation bioassays conducted in 50-mL conical tubes ( C ), where a single gravid female ( n = 25) was specifically exposed to either sterile water or water inoculated with one of the two tested yeast concentrations to enable the evaluation of stimulant or deterrent effects of yeast-derived waterborne substances. C Number of eggs laid by each female according to each type of oviposition substrate. Statistically significant differences between groups were identified with the Tukey post hoc tests. Columns labelled with different letters or with an asterisk (*) are significantly different with a p -value < 0.05. Columns labelled with a dot (•) are almost significantly different with a p -value < 0.1

Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

Techniques: Sterility, Derivative Assay

Journal: Microbiome

Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

doi: 10.1186/s40168-025-02099-6

Figure Lengend Snippet: Volatile organic compounds emitted by yeast species significantly affecting larval development and oviposition behavior of gravid mosquito females. Each yeast culture was prepared in CYM medium at a final volume of 100 mL and an initial cell concentration of 3.10 5 cells.mL. −1 (i.e., concentration shown to significantly impact both larval development and oviposition behavior). Cultures were incubated under constant agitation (130 rpm) for 18 h prior VOC analysis. A Heatmap illustrating the relative abundance of emitted VOCs detected by HS-SPME/GC–MS from five independent replicates of each yeast culture. B , C Relative abundances of 3-methyl- 1-butanol and 2-methyl- 1-butanol, two microbial VOCs shared by both attractive ( R. mucilaginosa and A. pullulans ) and repellent ( M. asiatica and T. delbrueckii) yeast species. The median is indicated by a horizontal black line. Statistically significant differences between yeasts, labelled with different letters, were determined using Dunn’s test with Bonferroni-Holm correction ( p -value < 0.05)

Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

Techniques: Concentration Assay, Incubation, Gas Chromatography-Mass Spectrometry